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Expression of Recombinant BPI_(23)-Fc γ1 Fusion Protein in CHO Cells

XU Jun\|Jie\+*\ XU Jing\ TONG Yi\|Gang\ WANG Hai\|Tao (Institute of Microbiology and Epidemiology, Beijing 100071, China)  
The fusion gene of BPI 23 and human Fcγ1 was obtained by PCR method,and the expression plasmid was constructed to express recombinant BPI 23 \|Fcγ1 fusion protein in CHO cells.After transfection with the plasmid and selection by methotrexate,the cell lines expressing the fusion protein were obtained.The recombinant protein was purified using cation\|exchange chromatography and its bioactivity was proved with bactericidal assays.
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