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《Chinese Journal of Biotechnology》 2001-05
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The Effects of Different Glucose Feeding Modes on hEGF Production in an Excretory Recombinant Escherichia coli K12 System

XU Zhi\|Nan\+1\ CEN Pei\|Lin\+1\ W K R Wong\+2 1(Dept.of Chemical Engineering and Bioengineering,Zhejiang University,Hangzhou\ 310027,China) 2(Dept.of Biochemistry,The Hong Kong University of Science & Technology,Clear Water Bay,Kowloon,Hong  
The effects of different glucose feeding modes on hEGF expression were evaluated in an excretory recombinant \%E.coli\% K12 system.The results showed that,compared with batch cultivation,the plasmid stability and density of plasmid\|retaining cells were improved by all three glucose feeding modes (intermittent,pH\|stat and constant\|rate).It was shown that hEGF yields were improved up to 25.5% and 28.1% by intermittent or pH\|stat glucose feeding respectively.Especially,up to 150% improvement of hEGF production was achieved by constant feeding of 200g/L glucose solution at a rate of 0.11 mL/min.The effects of further combined feeding with other medium components (ampicillin,nitrogen sources,and inorganic salts) and inducer on hEGF yield were also examined in the bench\|top fermentor.
【CateGory Index】: Q78
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