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《Chinese Journal of Biotechnology》 2002-01
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Hairy-root Culture of Polygonium multiflorum Thunb.and the Production of Its Active Constituents-anthraquinones

WANG Li\+1\ YU Rong-Min 1*\ ZHANG Hui\+1\ CHENG Ke-Di\+2 1(Laboratory of Biotechnology,Shenyang Pharmaceutical University,Shenyang\ 110016,China) 2(Institute of Materia Medica,Chinese Academy of Medical Science,Beijing\ 100050,China)  
Transformed hairy roots of \%Polygonium multiflorum\% Thunb.were obtained by the transformation of \%Agrobacterium rhizogenes\% LBA9402.It was clearly demonstrated that T-DNA of \%A.Rhizogenes\% Ri plasmid was integrated into the cells of hairy roots by the experiments of PCR and Southern hybridization.Through the screen of basic medium and the research of growth curve of hairy roots,the optimum inoculum time was selected in 30 days or so in the optimum condition-MS medium.On the condition,the content of rhein was 2.85 folds higher in hairy roots than that of natural plants by means of HPLC.
【CateGory Index】: Q943.1
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