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《Chinese Journal of Biotechnology》 2002-03
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Construction of Eukaryotic Expression Vector Using Neomycin-resistanceGene Mutant as Selectable Marker

GAO Chuan 1* ZHU Xu-Dong 2 ZHOU Xiao-Wei 2 YU Fang 2 LU Bai-Song 2 HUANG Pei-Tang 2 1(Institute of Pharmaceutical Chemistry, Beijing 102205,China) 2(Institute of Biotechnology, Beijing 100071,China)  
Neomycin-resistance gene is widely used as a selectable marker in eukaryotic expression vector. It codes neomycin phosphotransferaseⅡ(NPTⅡ) which confers resistance to various aminoglycoside antibiotic such as G418 and kanamycine. In this work ,by site-directed mutagenesis the neo gene mutant was obtained. The expression vector pmDNA using the neo gene mutant as selectable marker has been constructed. After inserting interest luciferase gene, the expression plasmid pmDNAluc+ was stably transfected CHO-K1 cells. As a result, the expression positive ratio reaches to approximate 95% and the ratio of high expression colonies is apparently higher than the controls.
【CateGory Index】: Q782
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【References】
Chinese Journal Full-text Database 1 Hits
1 Zhao Chuanjiang, Zhang Jincai, Xu Chenrong,et al.Department of Periodontology and Oral Medicine, Guanghua College of Stomatology, Sun Yat-sen University, Guangzhou 510055, China;Bioassay of the soluble human interleukin-1 receptor Ⅰrecombinant plasmid expression in vitro[J];Journal of Practical Stomatology;2006-06
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 MIAO Yun-gen (College of Animal Sciences, Zhejiang University, Zhejiang Hangzhou 310029, China);Insect Transposon and Its Application in Silkworm Gene Transformation[J];Bulletin of Sericulture;2004-02
2 Yi Yongzhu 1 Chen Yin 1 Zhang Zhifang 1 He Jialu 1 Qin Jian 2( 1Key Laboratory of Silkworm Biotechnology,Ministry of Agriculture,Sericultural Research Institute, Chinese Academy of Agricultural Sciences,Zhenjiang 212018; 2Sichuan Huashen Company,Chengdu 610075);Construction of the Host Range Expanded Recombinant-Rescure Inscet Baculovirus Expression Vector and the Expression of Foreign Gene[J];Acta Sericologica Sinica;2002-04
3 CAO Guang-Li XUE Ren-Yu HE Ze ZHENG Xiao-Jian SHEN Wei-De GONG Cheng-Liang~*(College of Life Sciences,Soochow University,Suzhou Jiangsu 215123,China);Research on hGM-CSF Transgentic Silkworm With piggyBac Transposon[J];Science of Sericulture;2006-03
4 ZHOU Wen-Lin WANG Chong-Long LIU Bo CAO Guang-Li XUE Ren-Yu SHEN Wei-De GONG Cheng-Liang*(College of Life Science,Soochow University,Suzhou Jiangsu 215123,China);The Elementary Transgene Research of Silkworm Cells Mediated by Transposon piggyBac[J];Science of Sericulture;2007-01
5 Chen Xiu, Zhang Feng *, Zhao Yun *, Qi Guorong *, Lu Changde *, Huang Junting (Sericulture Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang 212018) ( *Shanghai Institute of Biochemistry, Academia Sinica, Shanghai 2000;Application of Luciferase Report Gene in Transgenic Silkworm[J];HIGH TECHNOLOGY LETTERS;2000-01
6 Zhao Yun, Zhang Feng, Chen Xiu , Xu Anying , Feng Xiaoli, Huang Junting , Lu Changde (Institute of Biochemistry, Academia Sinica, Shanghai 200031) ( Sericulture Research Institute, Chinese Academy of Agricultural Science s, Zhenjiang 2120;Transgenic Silkworm with Green Fluorescence[J];HIGH TECHNOLOGY LETTERS;1999-06
7 LI Peng\+*, XU Yan, ZHANG Yunhui, et al.(\+*Department of Oral and Maxillofacial Surgery, West China College of Stomatology,Sichuan University, Chengdu 610041, China)\;;Construction of Eukaryote Expression Vector Carrying Human Soluble Interleukin-1 Receptor Gene[J];West China Journal of Stomatology;2003-02
8 XU Yan+*, LI Peng, ZHANG Yunhui, et al. (+*Department of Oral Medicine, College of Stomatology, Nanjing Medical University, Nanjing 210029, China);Expression of Constructed Eukaryotic Vectors Carrying Encoding Gene of Soluble Human Interleukin-1 Receptor in Mammalian Cells[J];West China Journal of Stomatology;2003-06
9 DENG Xiao zhao 1, ZHU Fan xiu 2, DIAO Zhen yu 1, GAO Jian 1, QI Yi peng 2 (1. Department of Molecular Biology,Nanjing Medical Institute of Nanjing Military Region,Nanjing 210002,Jiangsu,China. 2.Institute of Virology,Wuhan University);Expression of report gene in insect cells by a new tranfer vector with baculovirus early promoter[J];Bulletin of Medical Postgraduate;2001-03
10 GUO Xiu Yang, ZHOU Ze Yang  , FENG Li Chun, WANG Lin, LU Cheng, XIANG Zhong Huai (The Key Sericulture Laboratory of Agricultural Ministry, College of Sericulture &Silk, Southwest Agricultural University,Chongqing 400716,China);Sperm-mediated Gene Transformation of Silkworm[J];Progress In Biochemistry and Biophysics;2001-03
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