Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Chinese Journal of Biotechnology》 2002-03
Add to Favorite Get Latest Update

Cloning and Expression of the Cecropin B-thanatin Hybrid Antimicrobial Peptide in Escherichia coli

WENG Hong-Biao 1, 2* NIU Bao-Long 2 MENG Zhi-Qi 2 XU Meng-Kui 1 1(College of Animal Sciences, Zhejiang University, Hangzhou 310029, China) 2(Institute of Sericultural Sciences, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021,  
A 44-residue hybrid peptide (CB (1-24)-Arg-Ser-Tyr-Tan (4-21)) incorporating 1-24 residues of cecropin B (CB) and 4-21 residues of thanatin (Tan) was designed and constructed. The CB-Tan gene was cloned into expression plasmid pGEX-3X and expressed in E.coli BL21. The fusion protein was purified by affinity chromatography. After digested with enterokinase the gene product released with antibacterial activity and gave one band in Tricine-SDS-PAGE.
【Fund】: 浙江省重点项目资助 (No .2 0 0 2C2 2 0 19)~~
【CateGory Index】: Q785;Q786
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved