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Cloning of Schistosoma japonicum Chinese Strain 22.6kD Membrane associated Protein (Sj 22.6) Gene and Its Overproduction in Escherichia coli *

Cai Xuezhong 1 Lin Jiaojiao 1 Yang Guanzhen 2 Shi Fuhui 1 Shen Wei 1 Cai Youmin 1 Wu Xiangfu 2 1(Shanghai Institute of Animal Parasitology,Chinese Academy of Agricultural Sciences,Shanghai 200232) 2(Shanghai Institute of Bio  
A 576bp DNA fragment was amplificated by RT PCR,from Schistosoma japonicum adult worm mRNA.Sequence analysis revealed that this fragment contained S.japonicum Chinese strain membrane associated protein (Sj 22.6) gene.Then this gene was cloned into the expression vector pGEX 4T,and subsequently expressed in Escherichia coli. The recombinant GST fusion protein was purified by glutathione agarose affinity chromatography.Its molecular weight was about 48kD.The yield of expression was around 40mg/L E.coli culture.The immunological test suggested that the recombinant protein had good antigenity,which could make a good basis for the research of its immunological function in Schistosomiasis.
【Fund】: 国家生物高技术研究发展计划项目 总理专项基金
【CateGory Index】: Q78
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