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Human Erythroprotein Expressed in the Milk of Transgenic Mice *

Pan Ling 1 Xu Chong 4 Li Guangsan 2 Du Miao 2 Lao Weide 2 Chen Changqing 3 1(Shanghai Institute of cell Biology,The Chinese Academy of Sciences,Shanghai 200031) 2(Institute of Development of Biology,The Chinese Academy of Scienc  
The hybrid gene construct is composed of the 5' regulatory region of the goat β lactoglobulin (β LG) and human erythropointin genomic DNA sequences.After the gene transfer into mous by microinjection of DNA into fertilized eggs,the 4 transgenic mouse were identified by PCR,dot blot and Southern hybridization.The expression of Epo was detected in the milk of 3 nursing transgenic females of them by Epo ELISA analysis.The 4 transgenic mice produced offspring,part females of them were analysed by PCR and Southern hybridization,6 females were demonstrated carrying the transferred gene.Only two of them,Epo expressed at 0.5μg/mL in the milk detected by Dot ELISA.Our results show that 867 bp of goat β LG could direct Epo expressed exclusively in the mammary gland of transgenic mice.
【Fund】: 国家“八·五”羊个体表达基金
【CateGory Index】: Q26
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