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《CHINESE JOURNAL OF BIOTECHNOLOGY》 1999-01
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Studies on Xylanase Produced by Aspergillus ustus

Zhu Jing Wang Hao Liang Gaiqin Yan Zizheng (Institute of Microbiology,The Chinese Academy of Sciences,Beijing 100080)  
Aspergillus ustus showing high xylanase activity was isolated from 1144 strains of fungi.The medium for the optimal xylanase production(4% wheat spelt,0.5% glucose,0.4% NaNO 3 and 0.1% NaCl)yielded 2176 u/mL within 6 days of cultivation at 30℃.The enzyme showed a temperature optimum of 55℃ and retained 35% of activity after incubation at 45℃ for 1 h.The enzyme also showed a pH optimum of 5.5 and was stable in the range of pH 5.0 to 8.0.The K m value and V max value calculated were 4.3×10 -3 g/mL and 4.9 mg/(mL·min) for birchwood xylan,respectively.The hydrolysis products from birchwood xylan were xylotriose and xylotetraose.Thus,the enzyme is considered to be an endoglycosidese.
【Fund】: 国家“九·五”科技攻关计划
【CateGory Index】: TQ925
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