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First Strand Synthesis of cDNA by Application of Thermus aquaticus DNA Polymerase

Zhang Yindong Peng Cunzhi Zeng Xiansong Zheng Xueqin (National Key Biotechnology Laboratory for Tropical Crops,Haikou 571101)  
First strand synthesis of cDNA was catalyzed by application of the Taq DNA polymerase,then PCR was made using cDNA-RNA as a template with speciale primer.The results showed that the effects of first strands synthesis of cDNA catalyzed by Taq DNA polymerse and AMV Reverse Transcriptase were the same as each other,and ,could obtain longer cDNA first strands than AMV Reverse Transcriptase.These results are the first report in native.
【CateGory Index】: Q756
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1 LIU Li~1 CHEN Ji-Shuang~(2**) (School of Life Sciences,Huzhou Teachers College,Huzhou 313000)~1 (School of Life Sciences,Zhejiang Sci-Tech University,Hangzhou 310018)~2;Direct Amplification of Target Sequences by Taq DNA Polymerise Using Double-stranded Rna as Templates[J];Microbiology;2007-01
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