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Somatic Embryogenesis of Echinodorus orisis L.and the Kinetic Changes of the Endogenous Hormones Contents During the Embryogenetic Process

Xing Denghui Zhao Yunyun Huang Chenfen (Department of Biology,Capital Normal University,Beijing 100037)  
Somatic embryogenesis was achieved from young leaf cuttings of Echinodorus orisis L.,an aquatic ornamental plant,in a short period(25days).Among the cytokinins and their combinations tested,6 BA(1mg/L) and Zt(1mg/L) in MS medium induced the highest efficiency(100%) of somatic embryogenesis,with a maximum of 4.87 embroids per explant.Roots instead of somatic embryo were formed when NAA(0.5mg/L) was added to MS medium containing Zt(1mg/L).Matured embryoids were germinated and rooted in MS medium with IAA(1mg/L) after 5 days cultivation.Seventy two percent of the rooted plantlets transplanted survived in an aquarium.The endogeneous hormone contents in various stages of somatic embryogenetic process were measured by HPLC.The concentrations of all hormones tested were about 2 times that of CK,the cuttings from the untreated fresh leaves,after 10day of incubation.Meanwhile,the concentration of IAA occurred two peaks after 10 and 25 day cultivation respectively.The cytokinin (Zt and ZR) peak,about 8 times more than CK,appeared in 15 days cultivation when the heart shaped embryo had been formed.The fluctuation of the GA 3 concentration was very similar to that of cytokinin.The ABA,however,remained a stably quite high concentration after 10 days cultivation.
【CateGory Index】: Q942
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