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《Shanghai Medical Journal》 2004-01
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Detection on mutation of Ki-ras gene of human gastric cancer xenografts in nudemice

LIU Jing. The 2nd Oncology Department, Hospital Traditional Chinese Medicine of Shanghai, Shanghai 200071, China  
Objective To detect the mutation of Ki ras gene exon 12, 13 of human gastric cancer xenografts in nude mice. Methods A human gastric adenocarcinoma cell line SGC 7901 was grafted under splenic capsule of 32 mice, Ki ras gene exon 12, 13 were detected by PCR DNA method 12 weeks after the procedure. Results No Ki ras gene exon 12, 13 mutations were detected. Conclusions The presence of mutation of Ki ras gene exon 12, 13 on human gastric cancer xenografts of nude mice can not be confirmed.
【CateGory Index】: R735.2
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