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《Shanghai Medical Journal》 2007-01
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Construction of ubiquitinated vaccine against type 2 herpes simplex virus

XU Jinhua , XU Yan , ZHENG Zhizhong , XIA Tian , WANG Jian , LIANG Jun , ZHANGZhen , ZHUXiaohua , ZHUNaishuo. Department of Dermatology , Huashan Hospital, Fudan University , Shanghai 200040, China  
Objective To construct the DNA vaccine against type 2 herpes simplex virus(HSV-2) modified by ubiquitinated infectious cell protein 27(ICP27). Methods Ubiquitions and HSV-2 ICP27 377-459 gene sequences obtained by PCR amplification were fused. The fused gene was identified and then cloned into eukaryotic expression vector pcDNA3. 1, for obtaining recombinant vector UB-pcDNA3. 1 which was comfirmed by DNA sequencing. Results DNA sequence analysis showed the rate of homology was 100% comparing with the sequence from Genebank. Conclusions The DNA vaccine against HSV-2 modified by ubiquitination of ICP27 has been successfully constructed.
【Fund】: 上海市科委资助课题(024119011)
【CateGory Index】: R392
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Chinese Journal Full-text Database 1 Hits
1 ZHOU Peng1,2,LIU Kun2,WANG Xi-liang2,YU San-ke1 (1.College of Veterinary Medicine,Northwest A & F University,Yangling,Shaanxi,712100,China;2.State Key Laboratory of Pathogen and Biosecurity,Institute of Microbiology and Epidemiology,Academy of Military Medical Science,Beijing,100071,China);Construction of DNA Vaccine Against Herpes simplex virus by Using the Fusion Gene of Herpes simplex virus Type Ⅱ(HSV-2) Dominant Epitope and Hepatitis B Core Protein[J];Progress in Veterinary Medicine;2009-05
Chinese Journal Full-text Database 7 Hits
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