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《Journal of Shihezi University(Natural Science)》 2005-01
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Genomic DNA Extraction of Humulus Lupulus and Establishment of RAPD Reaction System

YUAN Jun-feng, ZHANG Xia, WANG Shao-ming, ZHANG Ling, XU Ji-ping(Key Laboratory of Oasis Ecology Agriculture of Xinjiang BINGTUAN,Shihezi Univesity,Shihezi,Xinjiang 832003,China)  
Two methods were adopted to extract genomic DNA for RAPD analysis from Humulus lupulus.The results showed that the method of low pH extraction medium with high salts was better than that of CTAB.The DNA templates of Humulus lupulus extracted from young leaves were amplified in RAPD.The effects of contents of Mg~(2+),dNTP,Taq DNA polymerase and DNA templates on experimental results were tested and the optimal reaction system of RAPD for Humulus lupulus as determined.
【Fund】: 教育部科学技术研究重点项目资助 ( 0 3 1 6 1 )
【CateGory Index】: S571.9035.3
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