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《Chinese Journal of Neuroanatomy》 2014-01
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mGluR5 specific agonist CHPG promotes proliferation of human neural stem cells

Zhao Lingyu;Jiao Qian;Guo Bo;Yang Pengbo;Chen Xinlin;Liu Yong;Institute of Neurobiology,Xi'an Jiaotong University College of Medicine;Department of Genetics and Molecular Biology,Xi'an Jiaotong University College of Medicine;  
Objective: To investigate the effects of metabotropic slutamate receptor mGluR5 specific agonist CHPG on the proliferation of human embryonic cortical neural stem cells( NSCs) and the related molecular mechanism. Methods:MTT assay and diameter measuring of neurospheres were used to analyze the effects of mGluR5 agonist CHPG on proliferation of human embryonic NSCs. The effects of mGluR5 agonist CHPG on the cell cycle and apoptosis of human NSCs were analyzed with flow cytometer. The expression of phosphorylated ERK,JNK and p38 MAPKs in human NSCs were examined with Western Blot after mGluR5 agonist CHPG treatment. Results: mGluR5 agonist CHPG promoted proliferation of human embryonic cortical NSCs. The number of cells in S and G2 / M phass significantly increased in CHPG groups compared to that of the control group( P 0. 05),meanwhile the number of cells in G1 / G0 phase remarkably decreased( P 0. 05); the percentage of early and late apoptic cells significantly decreased in CHPG groups( P 0. 05). It was found that p-ERK1 /2 and p-JNK2 levels significantly increased in agonist CHPG group compared to that of control group,but p-p38 remarkably decreased( P 0. 01). Conclusion: mGluR5 agonist CHPG promotes proliferation of human embryonic cortical NSCs with activation of mitogen-activated protein kinases signaling pathway.
【Fund】: 国家自然科学基金(81070998)
【CateGory Index】: R363
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【Citations】
Chinese Journal Full-text Database 1 Hits
1 Qian Xiaodan,Luo Chunxia,Zhu Dongya(Lab of Cerebrovascular Diseases,School of Pharmacy,Nanjing Medical University,Nanjing 210029,China);The Research Progress of Neural Stem Cell Transplantation[J];Chinese Journal of Cell Biology;2012-03
【Co-citations】
Chinese Journal Full-text Database 4 Hits
1 Wang Shiqiang,Lou Shujie(College of Exercise & Sports Science,Shanghai University of Sports,Shanghai 200438,China);Signaling pathway analysis on the differentiation of neural stem cell under normoxia and hypoxia[J];Chinese Journal of Neuroanatomy;2013-02
2 WANG Xiliang1 ZHAO Yan1 XIAO Yulong1 BAI Lijie2 1.Department of Thoracolumbar Spine Surgery,the Second Affiliated Hospital of Inner Mongolia Medical University,Inner Monggol Autonomous Region,Hohhot 010030,China;2.Department of Rheumatology and Immunology,the Affiliated Hospital of Inner Mongolia Medical University,Inner Monggol Autonomous Region,Hohhot 010059,China;Application and progress of neural stem cells and erythropoietin in spinal cord injury[J];China Medical Herald;2013-20
3 LI Chang-dong,SUN Jian-jun,LI Zhi-yun.(Lanzhou general hospital of PLA,Gansu,Lanzhou,730050,China);Migration and localization of umbilical cord mesenchymal stem cells labeled by DAPI in the traumatic brain injury rat models[J];Journal of Apoplexy and Nervous Diseases;2013-07
4 Fan Ming-chao 1 , Wang Qiao-ling 2 , Liu Ke 1 , Zhang Xin 1 , Guan Yun-qian 3 , Sun Peng 4 1 Department of Neurosurgical Intensive Care Unit, the Affiliated Hospital of Medical College, Qingdao University, Qingdao 266003, Shandong Province, China 2 Community Medical Service Center of Zhenjiang Road, Qingdao 266033, Shandong Province, China 3 Cell Therapy Center, Xuanwu Hospital Capital Medical University, Beijing 100053, China 4 Department of Neurosurgery, the Affiliated Hospital of Medical College, Qingdao University, Qingdao 266003, Shandong Province, China;In vitro culture of human embryonic striatum-derived neural stem cells[J];Chinese Journal of Tissue Engineering Research;2013-27
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