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Antioxidant and Antibacterial Activities of Various Seabuckthorn (Hippophae rhamnoides L.) Seed Extracts

P.S.Negi~1,A.S.Chauhan~2,G.A.Sadia~2,Y.S.Rohinishree~2,R.S.Ramteke~2(1.Human Resource Development Department,Central Food Technological Research Institute,Mysore 570 020,India;2.Fruit and Vegetable Technology Department,Central Food Technological Research Institute,Mysore 570 020,India)  
Seabuckthorn(Hippophae rhamnoides L.) seeds were successively extracted with chloroform,ethyl acetate,acetone and methanol(MeOH) using a Soxhlet extractor for 8h each.The crude extracts were screened for antioxidant and antibacterial activities.The reducing power and antioxidant activities evaluated in various in vitro models(1,1-diphenyl-2-picrylhydrazine and liposome model system) showed the highest activity for MeOH extract.The MeOH extract was also found to posses maximum antibacterial activity.The MIC values,with respect to MeOH extract for Bacillus cereus,Bacillus coagulans,Bacillus subtilis,Listeria monocytogenes,Yersiniaenterocolitica,were found to be 200,300,300,300,and 350 ppm,respectively.These results indicated the possibility of using seabuckthorn seeds for medicinal uses and food preservation.
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