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高效液相色谱法测定饲料中环丙氨嗪

High performance liquid chromatography (HPLC) with NH2 column and 97 % acetonitrile eluate to determine the cyromazine in feeds was described in this paper. The feed samples were extracted from acetonitrile containing 20 % NH3·H2O and concentrated. The calibration curves for cyromazine were linear in concentration range of 0.05 ~ 20.0 μg/mL(R = 0.99995).The recoveries at levels of 3.0,5.0,8.0 mg/kg fortified samples ranged from 87.23 % ~ 95.36 %,with coefficient of variation 3.04 % ~ 3.46 %. The limit of detection was 0.05 mg/kg.
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