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Application study on Bacillus subtilis-loaded maifan stone dietary additive in sea cucumber culture

Wen Zhixin;Sun Yongxin;Li Yajie;  
In this study,Bacillus subtilis-loaded maifan stone was used for larval sea cucumber(Apostichopus japonicus)dietary additive,to investigate its effects on weight gain rate and diseases resistance of sea cucumber.The experiment was divided into 4 groups:control group,Bacillus subtilis groups,maifan stone group,B.subtilis-loaded maifan stone group.After 60-day feeding trial,the results showed that:sea cucumber fed with B.subtilis-loaded maifan stone exhibited the highest weight gain rate,which increased by 22.80% compared with the group of control.Furthermore,a significant improvement of diseases resistances against Vibrio splendidus and Pseudoalteromonas nigrifaciens was also found in sea cucumber fed with B.subtilis-loaded maifan stone(P 0.05).The removal effects on ammonia nitrogen and nitrite in sea cucumber aquaculture waste water of the three additives were also investigated under the condition of nongas loading.The results showed that B.subtilis had no removal effect on ammonia nitrogen and nitrite in waste water,while maifan stone could remove ammonia nitrogen and nitrite in a short time(within 1 day).With respect to the long-term effect(within 7 days),B.subtilis-loaded maifan stone showed the optimal values.
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