Effects of copper sulfate and nano-copper on metabolism and development of broiler embryos
GUO Yongqing;ZHANG Xiaoyu;CHEN Yujie;WANG Yong;YANG Feng;Vocational and Technical College OF IMAU;Inner Mongolia Agricultural University Hohhot;
This study was conducted to investigate the in vitro injection of different dose of copper sulfate and nano-copper on metabolism and development of broiler embryos. A total of 240 aged 1 d special pathogen free(SPF)eggs were assigned to 6 groups,which had 4 replicates of 10 embryos. The control group was with no injection,and the negative control group was injected 0.25 mL deionized water,while the copper sulfate groups injected 50 and 100 mg/kg copper sulfate,and the nano-cooper group injected 0.25 mL 50 and 100 mg/kg nano-cooper. The embryos incubated for 21 d. The injection of 50 mg/kg nano-cooper caused particularly and significantly higher(P 0.05)oxygen consumption on 16 and 18 d compared with all other groups. The injection of 100 mg/kg nano-copper had higher oxygen consumption than the 50 mg/kg nano-copper group(P 0.05)on 12 and 14 d,while 100 mg/kg copper sulfate had higher oxygen consumption than the 50 mg/kg copper sulfate group on all stages(P 0.05). Compared with the 100 mg/kg nano-copper group,the energy consumption significantly increased in 50 mg/kg nano-copper group(P 0.05). The negative control and nano-copper groups had higher weight of residual yolk-sac than the copper sulfate group(P 0.05),while 50 mg/kg nano-copper had higher weight of residual yolk-sac than the 100 mg/kg nanocopper group(P 0.05). There was a significant difference in the fibroblast growth factor geneexpression forthe 100 mg/kg copper sulfate group compared with the other groups(P 0.05). We found that the in vitroinjection of 50 mg/kg nano-copper stimulated the metabolic rate of broiler embryos and the nano-copper had no harmful for the incubation of chicken embryos.
【CateGory Index】： S831.5