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《Life Science Research》 2017-06
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Effects of Over-expression of Secreted Frizzled-related Protein 5(SFRP5) on Glucose Metabolism in Mouse Liver

ZHU Wei;ZHENG Shi-xia;KE Lin-qiu;WANG Yi;CHEN Qiong-ke;XIA Dao-han;WANG Hong-man;Department of Endocrine and Integrative Medicine, Chongqing People's Hospital;  
Secreted frizzled-related protein 5(SFRP5) is one of anti-inflammatory lipid mediators, and is found to play regulatory roles in obesity-induced diabetes. Herein, four groups of eight-week-old C57BL/6J mice were randomly assigned as normal fat diet(SD)-empty adenovirus(Ad-GFP)(n=10), normal fat diet(SD)-adenovirus over-expression SFRP5(Ad-SFRP5)(n =10), high fat diet(HFD)-empty adenovirus(Ad-GFP)(n=10), and high fat diet(HFD)-adenovirus over-expression SFRP5(Ad-SFRP5)(n=10). After 12 weeks, phosphoenolpyruvate carboxykinase(PEPCK) and glucose-6-phosphatase(G6Pase) mRNA and protein expression in the mice livers were measured by real-time PCR and Western-blot, respectively. The phosphorylation of insulin signaling pathway was measured by Western-blot. The results showed that both mRNA and protein expression levels of PEPCK and G6Pase were significantly decreased(P0.05), and the levels of phosphorylation of insulin receptors(InsR) and protein kinase B(AKT) were significantly increased in HFD-Ad-SFRP5 group(P0.05). Thus, over-expression of SFRP5 in C57BL/6J mice may improve insulin resistance in the liver by decreasing hepatic gluconeogenesis and enhancing the insulin signaling pathway.
【Fund】: 重庆市基础与前沿研究计划一般项目(cstc2014jcyjA10112)
【CateGory Index】: R587.1
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