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Study on gene spectrum of low dose As_2O_3 exposed BEAS-2B cells

YU Ping;SUN Dao-yuan;LI Xue-fei;ZHOU Cai-cun;Department of Intoxation and Department of Oncology,Shanghai Pulmonary Hospital,School of Medicine,Tongji University;  
Objective To investigate differential the changes of gene expression in low-dose As2O3 exposed human bronchial epithelial cells( BEAS-2B),thereby providing the basis for the mechanism research on carcinogenesis by arsenic. Methods BEAS-2B cells were divided into two groups,the cultural solution of experimental group was added As2O3,the final concentration should reached 0.1 μg / L,while the control group cultural solution was only added an equal volume of normal saline solution. 3 months later,the total RNA of BEAS-2B cells from both group were extracted the cDNA by reversed transcription was labeled with Cy5 which then hybridized with whole genome gene expression profiling of OneArraychip. The data were treated with Rosetta ResolverSystem( Rosetta Biosoftware). Results The results showed that 1518 genes were found up-regulated among 30275 spotted microarrays, and 15 tumor related genes such as mitogen activated protein kinase( MAPK), v-ets erythroblastosis virus oncogene homolog 1( ETS1),insulin like growth factor binding protein 7( IGFBP7) etc.,additionally, 7 tumor related gene sets were found up-regulated,too. Besides,there were 1409 gene fragments were shown significantly lowered,such as apoptosis factor( BCL2L11) and cytochrome C oxidase subunit( COX5A),the only 2 tumor related pathway genes,and two tumor related gene sets were found down-regulated,too. Conclusion The results suggest that the up-regulation of some cancer genes may be an important regulation link of As2O3 inducing carcinogenesis in BEAS-2B cells.
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