Study on gene spectrum of low dose As_2O_3 exposed BEAS-2B cells
YU Ping;SUN Dao-yuan;LI Xue-fei;ZHOU Cai-cun;Department of Intoxation and Department of Oncology,Shanghai Pulmonary Hospital,School of Medicine,Tongji University;
Objective To investigate differential the changes of gene expression in low-dose As2O3 exposed human bronchial epithelial cells( BEAS-2B),thereby providing the basis for the mechanism research on carcinogenesis by arsenic. Methods BEAS-2B cells were divided into two groups,the cultural solution of experimental group was added As2O3,the final concentration should reached 0.1 μg / L,while the control group cultural solution was only added an equal volume of normal saline solution. 3 months later,the total RNA of BEAS-2B cells from both group were extracted the cDNA by reversed transcription was labeled with Cy5 which then hybridized with whole genome gene expression profiling of OneArraychip. The data were treated with Rosetta ResolverSystem( Rosetta Biosoftware). Results The results showed that 1518 genes were found up-regulated among 30275 spotted microarrays, and 15 tumor related genes such as mitogen activated protein kinase( MAPK), v-ets erythroblastosis virus oncogene homolog 1( ETS1),insulin like growth factor binding protein 7( IGFBP7) etc.,additionally, 7 tumor related gene sets were found up-regulated,too. Besides,there were 1409 gene fragments were shown significantly lowered,such as apoptosis factor( BCL2L11) and cytochrome C oxidase subunit( COX5A),the only 2 tumor related pathway genes,and two tumor related gene sets were found down-regulated,too. Conclusion The results suggest that the up-regulation of some cancer genes may be an important regulation link of As2O3 inducing carcinogenesis in BEAS-2B cells.