Cytotoxic effect of trichloroethylene on human immature dendritic cell
ZHAO Na;WU Qi-feng;LI Hong-ling;LIU Hao-zhong;WANG Hai-lan;Guangdong Provicial Center for Prevention and Treatment of Occupational Diseases;
Objective To study the cytotoxicity of trichloroethylene( TCE) on dendritic cells( DC). Methods Isolated human peripheral blood monocytes( PBMC) from healthy donors using magnetic activated cell sorting( MACS) technique were induced with rhGM-CSF and rhIL-4 to get immature dendritic cell( iDC),which were identified both using by detecting the surface markers with cell morphological observation and flow cytometry instrument( FACS) to detect cell surface marker. After treatment of TCE,the cells,Counting Kit-8( CCK-8) was used conduced to evaluate the cell viability of iDC treated by TCE and analyze its dose-effect and time-course studies effect relationships. Results It was showed that the induced iDC growed in a suspended status and some of them were clustered aggregated into clumps,majority with typical morphology round shape,the cytoplasm in some cells were found a little bit out of a small inconspicuous burr like dendrites. The cell surface marker expression positive rate were: There was high level expression simultaneously in CD1a( 85. 82%), HLA-DR( 50. 34%), and low level in CD14( 0. 35%), CD40( 23. 20%), CD80( 9. 65%), CD83( 25. 73%), CD86( 33. 67%),respectively. The number of iDCs showed decreasing trend in TCE treated group with TCE dose,the clustering tendency was gradually reduced,and the viability of iDC was significantly decreased too,with dose-and time-depended patterns( P 0. 05). Conclusion It was shown that there was some cytotoxic effect of TCE on human immature DC.