Relationship between DNA repair gene polymorphisms and DNA damage susceptibility in 1,3-butadiene exposed workers
LIU Nan;LI Bin;CHENG Juan;LI Yu;ZHENG Guo-ying;GUAN Wei-jun;CUI Tao;MENG Hui-lin;Laboratory of Occupational Health and Safety for Coal Industry,Hebei United University;
Objective To explore the relationship between DNA damage susceptibility to 1 3-butadiene( BD) and polymorphisms of DNA repair gene such as XRCC1,XRCC2,XRCC4 and RAD52. Methods 171 BD-exposed workers and 55 administrative personnels were selected as exposed subjects and controls respectively,in this study. DNA damage in peripheral lymphocytes was measured by alkaline comet assay,the polymorphisms of XRCC1,XRCC2,XRCC4 and RAD52 were detected by PCR-RFLP. Results The results showed that the comet tail moment of exposed group( 4. 53,3. 49—5. 87) was much higher than that of control group( 2. 38,0. 82—3. 98),there was significant defference( P 0. 01). Multivariate analysis of covariance further revealed that the comet tail moment of G28152A in BD exposed workers with XRCC1 AA genotype( 5. 18, 4. 23—6. 18) was significantly higher than that of GG genotypes( 4. 52,3. 43—5. 64,P 0. 05). Additionally,the comet tail moment of A245G in XRCC4 AA genotype( 4. 23,3. 35—5. 41) was significantly lower( P 0. 01) than that of AG( 5. 05,3. 79—6. 05) and GG genotypes( 5. 37,4. 29—6. 36). Conclusion The results suggested that genetic polymorphisms of XRCC1 G28152A and XRCC4 A245G might be related to the DNA damage induced by BD.