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《Food and Fermentation Industries》 2015-12
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Comparative study on DNA extraction from juice beverage

QI Ling-qian;LIU Xiu;DING Meng-xuan;LI Jing-yuan;LIU Yuan-yuan;YIN Jian-jun;China National Research Institute Food & Fermentation Industries;  
In order to obtain an efficient and general DNA extraction method from juice beverage,seven kinds of juice nectar and six kinds of clarified fruit juice were used as experimental material,the modified CTAB method was improved on pre-treatment,the composition of lysis buffer,the time of Isopropyl alcohol precipitation. Furthermore,we compared the extraction efficiency of CTAB,DNA extraction kit and modified CTAB method. The results showed that modified CTAB method was the most suitable for the DNA extraction from juice nectar and clarified fruit juice,for juice nectar,the concentration of isolated genomic DNAs was more than 24. 10 μg / m L and for clarified fruit juice,was more than 5. 7 μg / m L. Moreover,the value of OD260/ OD280 was close to 1. 8,and the isolated genomic DNAs also amplified137 bp size band of endogenous 18 S r DNA. Therefore,the best way of extracting DNA from juice is modified CTAB.
【CateGory Index】: TS255.44
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