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《Science and Technology of Food Industry》 2013-05
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Research of A. camphorata solid-state fermentation production Antroquinonol and extraction Antroquinonl

YU Xue-chun,XIA Yong-jun,ZHANG Huan,XU Gan-rong*,ZHANG Bo-bo (Key Laboratory of Industrial Biotechnology,Ministry of Education,School of Biotechnology,Jiangnan University,Wuxi 214122,China)  
Antroquinonol,a metabolite produced from solid-state fermentation of Antrodia camphorata has been shown great anti-cancer effect. This study investigated the effects of a number of factors including fermented grain types,substrate capacity,initial moisture content(containing the water of seeds),supplementary carbon source(glucose),nitrogen content on Antroquinonol content by Antrodia cinnamomea and the multi-stage countercurrent extraction method for extracting Antroquinonol from the solid-state fermentation products of Antrodia camphorata in order to improve the production of Antroquinonol in solid-state fermentation,the extraction efficiency,decrease loss and provide theoretical basis for preparation of Antroquinonol at industry scale. The results were as follows:the best compositions of fermentation medium for Antroquinonol content were as follows:rice 110g,initial moisture content 50%(with inoculated seed liquid water),carbon source 2.0g(glucose),nitrogen content 0. 3g(soybean powder). With these optimum conditions,Antroquinonol content increased by 14.44% than the initial value;After three-stage countercurrent extraction by 95% ethanol(volume ratio of ethanol and fermentation product was 10∶1,v/w)in 50°C water bath and shaking for 1h,the results showed that the residual rate of Antroquinonol in the fermentation products was less than 1%. This method showed the less solvent used and higher extraction efficiency.
【CateGory Index】: TQ920.6
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