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Antioxidant activities of polyphenols from pinus tabulaeformis pollen

FAN San- hong;QIN Wan- ning;FANG Chu- chu;College of Life Science,Shanxi University;  
The pinus tabulaeformis pollen polyphenols were extracted by ultrasonic with ethanol,and they were separated and purified by AB- 8 macroporous adsorption resin,the positive comparison was V_C ,the reducing power of pinus tabulaeformis pollen polyphenols and their scavenging effect on DPPH·,·OH,O_2~-·were researched by Fe~(3+)- reducing method,DPPH·reducing method,salicylic acid colorimetry and pyrogallol autoxidation method.The experimental results showed that when the concentrations both were 6 μg / m L,pinus tabulaeformis pollen polyphenols was 16.33% significant higher than that of V_C on DPPH·clearance( p 0.05),when the concentrations both were 8 μg / m L,pinus tabulaeformis pollen polyphenols was 32.03% significant higher than that of V_C on ·OH clearance( p 0.05),when the concentrations both were 6 μg / m L,pinus tabulaeformis pollen polyphenols was10.53% unsignificant higher than that of V_C on O_2~-· clearance.When the concentrations both were 10 μg /m L,the absorbance of pinus tabulaeformis pollen polyphenols was 0.712 at 700 nm,was significantly higher than that of V_C ( A_(700 nm)= 0.353667,p 0.05),so the reducing power of pinus tabulaeformis pollen polyphenols was higher than that of V_C . The results showed that pinus tabulaeformis pollen polyphenols had stronger reducing power and scavenging effectt on oxygen free radicals,and its clearance rate was higher than that of V_C . In conclusion,the pinus pollen polyphenols have significant antioxidant activity.
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