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Research of the Influence of the Contents of Caffeine in Different Dark Tea's Fungi Growing Process by RP-HPLC

Xia Li;Fang Fang;Liu Shiquan;Hunan City University;Hunan Provincial Key Lab of Dark Tea and Jinhua;  
To reveal the effect of dark tea fungus growing process on the content of caffeine in tea, the tea "floating" before and after tea samples as the research object, establish a method for the determination of caffeine in tea RP-HPLC, tea caffeine content changes were measured before and after flooding. Methods Hypersil ODS column was used for the gradient elution of methanol water phase. The flow rate was 1 m L/min and the detection wavelength was 272 nm. Methods the linear relationship was good, the precision was good, the accuracy was high, and the average recovery rate was 99.3%. The results showed that tea caffeine content and raw tea after flooding containing ratio decreased significantly.
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