Determination of Nonylphenol in Milk Powder and Liquid Milk by UPLC-MS/MS
Fang Ling;Shanghai Intertek Co.,Ltd.,(ITS);
The analysis method of nonylphenol in milk powder and liquid milk by UPLC-MS/MS was established. The isotopic internal standard of nonylphenol was added to milk powder and liquid milk after freeze-dried into powder, then the samples were extracted with cyclohexane and ethyl acetate and then cleaned up by GPC, purified water and methanol was used as mobile phase by isocratic elution, the dyes were analyzed by MS/MS in multiple reaction monitoring(MRM) mode and quantified by the Internal standard. The LOD of nonylphenol was 1 μg/kg, LOQ was 3 μg/kg, good linear relationship(R~2 = 0.995) was found in 0.5 ~ 10 ng/mL linear range, the recovery of nonylphenol was 80% ~ 95%, RSD 10%. The method is practical, rapid and accurate, which can be used to detect nonylphenol in milk powder and liquid milk.
【CateGory Index】： O657.63;TS252.7