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Optimization of Extraction Technology of Pineapple Peel Pigment by Response Surface Methodology

LIU Ming-shou,CHEN Fa-he,WU Guang-bin(College of Bio-Engineering,Jimei University,Xiamen 361021,Fujian,China)  
In order to achieve the comprehensive utilization of pineapple peel,the extraction process of pigment from pineapple peel was studied.By single factor and orthogonal tests,2 organic solvent extraction process route of both fresh and dry pineapple peel were determined.By contrast,the process route of dry pineapple peel was chosen and its process parameters were further optimized using the response surface method,the ultimate optimal process was:75 % ethanol as extraction solvent,solid and liquid ratio 1:60(g/mL),extraction temperature 70 ℃,degrees of fragmentation:through 10 meshes,the number of extraction cycles:2,extraction time of cycle 1:2 h,cycle 2:0.5 h.The products got by applying this process were as follow:oil-soluble pigment from pineapple peel,colour value:E1 %1 cm415 nm=9.031,its total content of carotenoids:3.149 mg/g;water-soluble pigment from pineapple peel,colour value:E1 %1 cm280 nm =7.413,its total content of flavonoids 2.451 mg/g;flavone from pineapple peel,its total content of flavonoids:245.966 mg /g.
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