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《Food Research and Development》 2011-12
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Optimization of Extraction Process of Polysaccharides from Elatostema stewardii Merr by Response Surface Methodology

YANG Jian-yuan,ZHOU Xiao-ling,WANG Ping-lan,YUAN Zhi-pin,XU Wen-quan,LI Yue-ping(College of Life Sciences,Jiujiang University,Jiujiang 332000,Jiangxi,China)  
To optimize extraction process of polysaccharides from Elatostema stewardii Merr.According to single-factor experiments,effects of extraction temperature,extraction time and ratio of water to material as well as their interactions on yield of the polysaccharides were studied by central composite design.The predictive polynomial quadratic equations model was developed by response surface analysis.The optimum extraction conditions of the polysaccharides from Elatostema stewardii Merr were obtained as follows: extraction temperature 96 ℃,extraction time 4.4 h and liquid/solid ratio 42∶1(mL/g),which led to estimated and observed values of maximal yield of polysaccharides of 13.86 % and 14.06 %,respectively.
【CateGory Index】: TQ461
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