Research on Antioxidant Effect of Total Flavonoid Extracts from Celery Seed in vitro
DU Juan;SONG Chun-Mei;ZHANG Lan;DANG Rui;Jilin Medical College;
The flavonoid in celery seeds were extracted by the method of methanol reflux and the total flavonoid in extraction were determined with VC as control group. The inhibition ratio to DPPH·, hydroxy free radical(·OH)clearance and total antioxidant ability of total flavonoid in extraction were determined by the methods of DPPH,Fenton and FRAP.The results showed that, in the determination of the concentration range, flavonoids in the Celery seeds could inhibit generation of DPPH· and ·OH, the flavonoids and inhibiton took on positive relativity, and with the extract of flavonoid concentration increased, the total antioxidant capacity also gradually strengthen. It was proved that celery seed flavonoids had strong antioxidant activity.