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《Journal of Shanghai Fisheries University》 2008-01
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Analysis on the composition of culturable bacterial communities in several tissues of puffer fish Takifugu obscurus

YANG Gui-mei1,BAO Bao-long1,LI Hui-rong2,TANG Wen-qiao1,REN Da-ming3(1.Key laboratory for Aquatic Genetic Resources and Aquacultureal Ecology Certificated by the Ministry ofAgriculture,Shanghai Fisheries University,Shanghai 200090,China;2.SOAKey Laboratory for Polar Science,Polar Research Institute ofChina,Shanghai 200136,China;3.State Key Laboratory ofGenetic Engineering,Fudan University,Shanghai 200433,China)  
Using 16S rDNA PCR denaturing gradient gel electrophoresis (PCR-DGGE) technology,we investigated the composition of culturable bacterial communities in the skin,intestines,and TTX-accum ulating tissues (ovary,liver) of the obscure striped puffer fish Takifugu obscurus fed natural or artificial diets.Atotal of 45 species of different culturable bacteria were isolated and classified according to phylogenetic analysis.Of these species,those belonging to the class γ-Proteobacteria dominated the microbial community in the puffer fish,while others belonged to the group of low G +C Gram positive bacteria,and the group of high G +C Gram positive bacteria.Large differences in the bacterial assemblage isolated from the intestines,TTX-producing organs and skins of fish fed natural and artificial diets were observed.No matter fed with natural or artificial diets,the tissue of intestine,TTX-accumulating organ,or skin,was found to contain bacteria from TTX-producing genera reported to-date.Puffer fish restricted to artificial diets from birth still had large assemblages of TTX-producing bacteria,indicating thatfood chain accumulation of TTX contributes little to the toxicity of these fish.
【Fund】: the Key Discipline funding Y1101 Key foundation program06dj14003 from Shanghai Municipal Science Committee China
【CateGory Index】: S917.1
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