Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Shanghai Fisheries University》 2008-01
Add to Favorite Get Latest Update

Relationship of white spot syndrome virus envelope protein VP31 with precociousness of WSSV

WANG Yong-jie,PANG Ting-shuang,LI Yan-he,SHE Lei(Institute of Fisheries,Anhui Academy of Agricultural Sciences,Hefei 230031,China)  
White spot syndrome virus(WSSV) is a devastating viral pathogen of cultured shrimp worldwide.In this paper,two primers were designed according to the sequences of envelope genes,VP31 of white spot syndrome virus(WSSV) in the GenBank.The DNA fragment about 783 bp amplified by PCR were linked to the BamHⅠand EcoR I restriction endonuclease site,then cloned into pFAST BAC HTB and expressed in the 5th instar larvae of silkworm.The molecular weight of the engineered protein was about 31 kD,which was identified by SDS-PAGE analysis.Antiserum of envelope protein VP31 purified by Ni2+ column chromatography was prepared for immunizing New Zealand rabbit.The VP31 antiserum was used to neutralize the WSSV infection on crayfish by intramuscular injection.A neutralization assay with this antibody demonstrated that envelope protein VP31 is involved in WSSV infection and that VP31 might play a key role during this process.
【Fund】: 国家重点基础研究973发展计划基金(2005CB121000);; 安徽省农科院院长创新基金(2006-76)
【CateGory Index】: S945
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved