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The construction of androgenic gland cDNA library of malaysian giant prawn Macrobrachium rosenbergii and the partial screening of expressed sequence tags

LIU Ping,QIU Gao-feng,ZHENG Liang,ZHANG Yu-ke(Key Laboratory of Aquatic Genetic Resources and Aquacultural Ecology Certificated by Ministry of Agriculture,Shanghai Fisheries University,Shanghai 200090, China)  
The androgenic gland is an endocrine gland that exists only in male Crustacea.It has been shown that the androgenic gland plays a critical role in sex differentiation.In order to obtain expressed sequence tags(ESTs) and possible full-length sequences of functional genes in the androgenic gland of Malaysian giant prawn Macrobrachium rosenbergii,a cDNA library of the androgenic gland was constructed by using the SMART technology.Partial screening was performed through the cDNA library by means of the colony PCR.Of 114 positive recombinants,24 clones were randomly picked up to sequence.The results revealed a total of 12 ESTs including 4 functional genes,4 rRNAs and 4 new genes with no homologies in the Genbank.The present research has a high ratio of full-length cDNA sequences using the technology of SMART and makes a primary conclusion for the potential functions of the four isolated known genes.Thus,it establishes the platform for further screening more functional genes related to the differentiation and development of the androgenic gland.
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