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Construction of PCR-DGGE gene fingerprint for the gastrointestinal bacterial community in Ephippus orbis

ZHOU Zhi-gang,SHI Peng-jun,YAO Bing,HE Su-xu(Feed Research Institute of Chinese Academy of Agricultural Sciences,Beijing 100081,China)  
The preliminary analysis of the bacterial community structure in the gastro-intestine of Ephippus orbis based on 16S rDNA PCR-DGGE fingerprint using a culture-independent approach was conducted in present study.The DGGE fingerprint showed that 7 obvious bands were found in the samples of stomach content,stomach wall,and gut wall of Ephippus orbis,and 6 bands in the sample of gut content,which might reflect the existence of rich bacteria species in the digestive tract of Ephippus orbis.The clustering analysis to the DGGE fingerprint displayed that the similarity of the microflora structures in the stomach content and gut content was highest(90%) and that in the stomach wall and gut wall was lower(80%).The self-quantity analysis showed that the coefficient variances of the relative abundances in the bacteria composition of the gastro-intestine in Ephippus orbis were larger than 47.4%,which suggested that one or few kind of bacterium predominated the digestive tract of Ephippus orbis.
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