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Genetic diversity in Parabramis pekinensis from the lower reaches of the Yangtze River from RAPD analysis and mitochondrial D-loop sequences

LI Jian-lin,TANG Yong-kai,YU Ju-hua(Key Open Laboratory for Genetic Breeding of Aquatic Animals and Aquaculture Biology,Ministry of Agriculture,Freshwater Fisheries Research Center,Chinese Academy of Fishery Sciences,Wuxi 214081,China)  
Genetic diversity in Parabramis pekinensis from the lower reaches of the Yangtze River was analyzed using RAPD and mtDNA D-1oop sequence.Twenty-eight primers were chosen from total 40 random primers for amplification in 24 individuals genomic DNA,total 178 RAPD sites were detected in these individuals,83 of 178 loci were polymorphic,which accounted for 46.63%.The sizes of DNA fragments were from 0.2 kb to 2 kb.The genetic distances between individuals were from 0.047 6 to 0.132 6,and average genetic distance was 0.088 5.The Shannon's index was 0.098 6.The RAPD results showed the genetic diversity of the Parabramis pekinensis population in lower reaches of the Yangtze River was in medium state.Sequences comparison of 938 bp mtDNA D-loop region from 8 individuals of Parabramis pekinensis revealed they were 8 haplotypes and the haplotypic diversity is 1.000.There were total 17 variation loci,which came from 15 transition sites,and 2 transversion sites in 938 bp D-loop region.The mutation rate was 1.81%,the nucleotide diversity is 0.0062,and the average number of nucleotide differences is 5.821.The average genetic distance of haplotypes is 0.0063.Sequences comparison of mtDNA D-loop displayed little genetic diversity of the Parabramis pekinensis population.
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