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Isolation and characterization of novel microsatellite markers from an enriched mud carp(Cirrhina molitorella) genomic library

YANG Cheng1,2,ZHU Xin-ping3,HAO Jun4,SUN Xiao-wen2(1.College of Aqua-life Science and Technology,Shanghai Fisheries University,Shanghai 200090,China;2.Heilongjiang River Fisheries Research Institute,Chinese Acadamy of Fishery Sciences,Harbin 150076,China;3.Pearl River Fisheries Research Institute,Chinese Acadamy of Fishery Sciences, Guangzhou 510380,China;4.College of Life Science and Technology,Dalian Fisheries College,Dalian 116023,China)  
A(CA)n enriched microsatellite library was constructed for mud carp Cirrhina molitorella using a magnetic beads enrichment procedure.After sequence analysis on 60 randomly picked colonies from 252 positive clones,56(93.3%) of the colonies were found to contain microsatellite sequences.Among the 56 microsatellites,49 were perfect(87.5%),2 were imperfect(3.6%) and 5 turned out to be compound(8.9%).18 pairs of PCR primers were designed through software package.Microsatellite variation was assessed using 20 mud carp individuals,and 12 microsatellite loci were turned out to be polymorphic.The number of alleles ranged from 2 to 20 and the expected heterozygosity(He) ranged from 0.261 5 to 0.950 0.The results of this work may contribute to future studies of parentage,population genetic structure and the construction of the genetic mapping for the mud carp.
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