Full-Text Search:
Home|About CNKI|User Service|中文
Add to Favorite Get Latest Update

Cloning and Sequence Analysis of cDNA encoding toll-like receptor in Litopenaeus vannamei

YE Min-yu1,LIU Li-ping1,DAI Xi-lin2,SHEN Mo-chen1,XU Can1 (1.Key Open Laboratory of Aquatic Genetic Resources Excavation and Utilization Jointly Constructed by the University,the Municipality and the Ministry of Education,Shanghai Fisheries University,Shanghai 200090,China;2.Key Open Laboratory of Aquatic Genetic Resources and Aquaculture Ecology by the Ministry of Agriculture,Shanghai Fisheries University,Shanghai 200090,China)  
In this paper,cDNA encoding Toll-like receptor was isolated from total RNA of haemocytes,hepatopancreas,muscle,gill of white leg shrimp(Litopenaeus vannamei).Degenerate primers were designed based on the highly conserved TIR domain.Sequence was compared with other species,including Penaeus monodon,Tribolium castaneum,Drosophila melanogaster,Apis mellifera,Caenorhabditis elegans and Strongylocentrotus purpuratus by constructing phylogenetic tree.The comparison showed that there is high similarity between the shrimp TIR domain and the known TIR sequences in other species.The paper supplies fundamental data for better understanding of the immune system of the shrimp Litopenaeus vannamei.
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©CNKI All Rights Reserved