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Identification and phylogenetic analysis of pathogen-Ewardsiela tarda from cultured turbot(Scophthalmus maximus)

YANG Chun-zhi,WANG Xiu-hua,HUANG Jie (1.Key Laboratory of Sustainable Utilization of Marine Fishery Resources,Ministry of Agriculture,Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Qingdao 266071,China;2.College of Life Science and Technology,Shanghai Fisheries University,Shanghai 200090,China)  
A strain of bacteria(strain number 1101) was isolated from diseased turbot(Scophthalmus maximus)in a fish farm in Shandong peninsula.Challenge test proved that the strain had higher virulence.Identifying methods including 16S rRNA sequence test,physiological and biochemical characteristics and phylogenetic analysis were employed to identify the strain 1101 respectively.The results show that strain1101 was Gram negative,oxidase negative,catalase positive. Result by API20E indicated that strain 1101 was Ewardsiela tarda with 99% reliability.A 1419 bp DNA was sequenced and compared with other bacteria in GenBank.Homology analysis and phylogenetic study showed that it has the highest similarity to Ewardsiela tarda with 99% similarity.According to morphological features,physiological and biological characteristics and 16S rDNA homology comparison of the bacteria,the pathogenic bacteria were Ewardsiela tarda.Drugs sensitivity tests showed that strain1101 has sensitive to polymyxin.While other drugs had no obvious inhibitory activity to the strain.
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