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The antagonistic property of lactic acid bacteria derived from Orange-spotted grouper(Epinephelus coioides) larvae

YANG Hong-ling1,SUN Yun-zhang1,2,YE Ji-dan1,2,CHANG Jian-bo1,2,CHEN Zheng-qiang1 (1.The Key Laboratory of Science and Technology for Aquaculture and Food Safety, Jimei University,Xiamen 361021,China;2.Xiamen Key Laboratory of Feed Detection and Safety Evaluation,Jimei University,Xiamen 361021,China)  
Three strains of lactic acid bacteria,Y4-2,EA-1 and Y1B-3,were isolated from orange-spotted grouper(Epinephelus coioides) larvae.Lactic acid production and antagonistic property of three strains were studied in vitro.Results showed that EA-1 and Y1B-3 exhibited more rapid production of lactic acid and the amount of lactic acid were higher significantly than that of Y4-2 in the late fermentation period(P0.05).Consequently,pH in the culture of EA-1 and Y1B-3 decreased faster than that of Y4-2.Culture supernatants of the three strains showed different degrees of antagonistic activity on the pathogenic Vibrio harveyi and Vibrio alginolyticus.However,lactic acid had no effect on the inhibition action of the three strains,suggesting that the bacteria produced other inhibitory substances apart from lactic acid.The inhibition effects of cultures supernatant were above 70% after 15 min of heat treatment at 60 ℃ or 80 ℃,but lost their inhibition effects after 100 ℃ treatment.The inhibition substances of three strains were susceptive to trypsin and showed higher antagonistic activity at near neutral condition.
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