Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Shanghai Ocean University》 2019-01
Add to Favorite Get Latest Update

Zebrafish tbx20 gene knockout using CRISPR/Cas9 system and its related functions analysis

ZHU Zhe;HU Peinan;LI Weiming;ZU Yao;International Research Center for Marine Biosciences,Ministry of Science and Technology,Shanghai Ocean University;Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education,Shanghai Ocean University;National Demonstration Center for Experimental Fisheries Science Education,Shanghai Ocean University;  
The tbx20 mutated zebrafish line is successfully established by using CRISPR/Cas9 system. The tbx20 mutagenesis efficiency is about 42. 1 % which was evaluated by T7E1 assay in F_0. Sequencing analysis estimated the germline transmission efficiency of tbx20 mutagenesis is 36. 7 % in F_1. The tbx20 homozygote mutant showed obvious cardiac phenotypes: the tbx20 mutant displayed pericardial oedema,sinus congestion,looping abnormal and the cardiac morphology deformation at 48 hpf; all the phenotypes became severer at 3dpf and tbx20 mutant showed linear heart. In situ hybridization and realtime fluorescence quantitative PCR results revealed that the expression of vmhc was upregulated,amhc and myl7 in the tbx20 mutants were downregulated. The tbx20 mutated zebrafish line was successfully produced and was confirmed tbx20 affected heart looping,which established a foundation for future studies on the function of tbx20 gene in early cardiac chamber differentiation.
【Fund】: 国家自然科学基金(31501166);; 上海市教育委员会晨光计划项目(14CG49)
【CateGory Index】: Q78
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved