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《Acta Ecologica Sinica》 2003-01
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Studies on relationship between the ecophysiological feature and leaf characteristics of cucumber

WANG Shao-Hui, ZHANG Fu-Man (Horticultural College, China Agricultural University,Beijing 100094,China).  
The characteristics of leaf stoma of different cucumber ecotypes were studied by scan electron microscope. The leaf selected for scanning electron microscopy were 1mm2, were fixed in 3mml, 1mol/L gluteraldehyde for 24 h, rinsed three times with cacodylate buffer (pH 7.2), then postfixed in osmium tetroxide for 2 h. Samples were removed from osmium tetroxide, washed there times in the cacodylate buffer, and dehydrared in a graded series of ethanol, later embedded in Spur's epoxy resin, attached to uncoated slide and stained with epoxy tissue stain.Then Sputter-coated with gold in a sputter coater. Sample were cut into halves, one was used for the observation of face leaf, the other was used for reversed leaf.Leaf surface morphology was examined with scanning electron microscope S-530. The stoma density was observed in low multiple, others were with high multiple.10 view fields were observed.The results were as followed: Difference of leaf stoma structure in different cucumber ecotypes was significant.The stomata density of Asia type(Xintaimici and Tiancuilv) is higher than that of European ecotypes(Marianna and Deltstar) and European-Asia hybrid ecotypes(Yushi 3 and 97W-19).The guard cell length of European ecotypes is longer than that of others ;there are many obvious deep concave-convex cubicula drapes around the guard cell and depressed center of the drapes in Asia ecotypes stoma,which will store CO 2 and H 2O for photosynthesis and reduce the transpiration;For European ecotypes (Marianna),the stoma stands out the flat cuticular ,which allow CO 2 and H 2O in and out freely , as for Deltstar, there is obvious protuberance around the stomata,the cuticular around the stomata is less flat than that of Marianna.As for European-Asia hybrid ecotypes Yushi 3 and 97W-19, the leaf characteristics are between the two ecotypes above.With the soil moisture decreased, the stomata density increased, but the guard cell size is smaller, and the stomata aperture is larger.
【Fund】: 国家自然科学基金项目 ( 39830 2 30 )~~
【CateGory Index】: S642.2
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