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《Progress in Modern Biomedicine》 2012-22
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Effect of Anthocyanin Extract of Mulberry Fruit on the Apoptosis and the Mitochondrial Membrane Potential of Breast Cancer Cells

CHANG Hui1,WANG Zhan1,YUAN Li-jia1,FU Yu-jie1,2,MI Man-tian1(1 Department of Nutrition and Food Hygiene,College of Preventive Medicine,the Third Military University,Chongqing Key Laboratory of Nutrition and Food Safety,Chongqing,400038,China; 2 School of Pharmacy and Bioengineering,Chongqing University of Technology,Chongqing 400050,China)  
Objective:To study the effect of anthocyanin extract of mulberry fruit on the apoptosis and the mitochondrial membrane potential of breast cancer cells MDA-MB-453,MDA-MB-231 and MCF-7.Methods:Preparation of anthocyanin-rich extract from mulberry fruits was carried out by ultrasonic extraction with acidified-ethanol.The breast cancer cells MDA-MB-453,MDA-MB-231 and MCF-7 were reated with 50,100 or 150 mg/mL of the anthocyanin-rich extract for 24h;the cells apoptosis were analyzed by Annexin V/PI dyeing and flow cytometric assay.To characterize the upstream factors involved in the intrinsic apoptosis pathway,the mitochondrial permeability of MDA-MB-453 cells were measured by JC-1 staining and the laser confocal scanning microscopy.Results:The apoptosis analysis results indicated that the anthocyanin-rich mulberry fruit extract treated for 24 h,the apoptosis rate of these three breast cells in 100 and 150 mg/mL treated groups markedly increased(P0.05),exhibiting a dose-effect relationship.The observation by laser confocal scanning microscopy suggested that the anthocyanin-rich extract significantly decreased the mitochondrial membrane potential of breast cancer MDA-MB-453 cell;the ratio of JC-1 red/green fluorescence was significantly decreased(P0.05).Conclusion:The anthocyanin-rich extract significantly decreased the mitochondrial membrane potential of breast cancer cells and induced apoptosis.
【Fund】: 国家自然科学基金(30901194);; 第三军医大学青年创新基金(2009XQN14)
【CateGory Index】: R737.9
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