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《Progress in Modern Biomedicine》 2012-22
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Effect of Anthocyanin Extract of Mulberry Fruit on the Apoptosis and the Mitochondrial Membrane Potential of Breast Cancer Cells

CHANG Hui1,WANG Zhan1,YUAN Li-jia1,FU Yu-jie1,2,MI Man-tian1(1 Department of Nutrition and Food Hygiene,College of Preventive Medicine,the Third Military University,Chongqing Key Laboratory of Nutrition and Food Safety,Chongqing,400038,China; 2 School of Pharmacy and Bioengineering,Chongqing University of Technology,Chongqing 400050,China)  
Objective:To study the effect of anthocyanin extract of mulberry fruit on the apoptosis and the mitochondrial membrane potential of breast cancer cells MDA-MB-453,MDA-MB-231 and MCF-7.Methods:Preparation of anthocyanin-rich extract from mulberry fruits was carried out by ultrasonic extraction with acidified-ethanol.The breast cancer cells MDA-MB-453,MDA-MB-231 and MCF-7 were reated with 50,100 or 150 mg/mL of the anthocyanin-rich extract for 24h;the cells apoptosis were analyzed by Annexin V/PI dyeing and flow cytometric assay.To characterize the upstream factors involved in the intrinsic apoptosis pathway,the mitochondrial permeability of MDA-MB-453 cells were measured by JC-1 staining and the laser confocal scanning microscopy.Results:The apoptosis analysis results indicated that the anthocyanin-rich mulberry fruit extract treated for 24 h,the apoptosis rate of these three breast cells in 100 and 150 mg/mL treated groups markedly increased(P0.05),exhibiting a dose-effect relationship.The observation by laser confocal scanning microscopy suggested that the anthocyanin-rich extract significantly decreased the mitochondrial membrane potential of breast cancer MDA-MB-453 cell;the ratio of JC-1 red/green fluorescence was significantly decreased(P0.05).Conclusion:The anthocyanin-rich extract significantly decreased the mitochondrial membrane potential of breast cancer cells and induced apoptosis.
【Fund】: 国家自然科学基金(30901194);; 第三军医大学青年创新基金(2009XQN14)
【CateGory Index】: R737.9
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【Citations】
Chinese Journal Full-text Database 6 Hits
1 CHANG Hui1,MI Man-tian1,GU Yan-yan1,YUAN Jia-lin1,LING Wen-hua2,LIN Hui1 (1Department of Nutrition and Food Hygiene,Chongqing Key Laboratory of Nutrition and Food Safety,College of Military Preventive Medicine,Third Military Medical University,Chongqing 400038;2Department of Nutrition,Public Health School,Sun Yet-sen University,Guangzhou 510080,China);Antiproliferative and proapoptotic effects of 3,6-dihydroxyflavone on different cancer cells[J];Acta Academiae Medicinae Militaris Tertiae;2009-16
2 Wang Zhan1,Fu Yujie1,2,Chang Hui1,Zhao Changjun1,Mi Mantian1(1Department of Nutrition and Food Hygiene,Chongqing Key Laboratory of Nutrition and Food Safety,College of Military Preventive Medicine,Third Military Medical University,Chongqing,400038;2School of Pharmacy and Bioengineering,Chongqing University of Technology,Chongqing,400050,China);Inhibitory effect of anthocyanin extract of mulberry fruit on growth of breast cancer MDA-MB-453 cells[J];Journal of Third Military Medical University;2011-10
3 LIU Xiao-hong1,SHEN Yang2△(1 The Second Medical College of Lanzhou University,Lanzhou,Gansu 730030,China; 2 the Second Hospital of Lanzhou University,Lanzhou,Gansu 730050,China);Effect of Clostridium DifficileToxin A on Proliferation and Apoptosis of FRH-0201 Cholangiocarcinoma Cell Line[J];Progress in Modern Biomedicine;2011-01
4 LI Wen-hai1, HU Yun-Sheng2, ZHOU Yong-An1, LEI Jie1, SU Kai1, LI Xiao-fei (1 Department of Thoracic Surgery, Tangdu Hospital, Fourth Military Medical University, XI'an 710038, P. R. China; 2 Department of Orthopaedics Surgery, Tangdu Hospital, Fourth Military Medical University, XI'an 710038, P. R. China);Molecular Mechanism of Sliybinin-induced Apoptosis of Lung Cancer Cell Anip973[J];Progress in Modern Biomedicine;2011-09
5 WANG Xiao-yang,ZHANG Yuan,ZHANG Zhi-qin,YU Yong,MAO Yu-fei The Jinhua medical college of vocational technique university,Jinhua,Zhejiang,321000;Preventive action of extract from mulberry sarcocarp on experimental hyperlipidemia[J];Chinese Journal of Cardiovascular Rehabilitation Medicine;2009-05
6 Li Dongxiang, Chen Qingxi(College of Horticulture, Fujian Agriculture And Forestry University, Fuzhou 350002);Study Progress of Functional Ingredient and Development in Mulberry[J];Chinese Agricultural Science Bulletin;2009-24
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 WANG Rui et al(Chemistry Department,Zhaotong Teacher's College,Zhaotong,Yunnan 657000,China);Study on the Free Radical Scavenging Activity of FRUCTUS MORI Polysaccharides in Vitro[J];Journal of Anhui Agricultural Sciences;2012-02
2 ZHAO Ming et al(School of Pharmacy,Jiangsu University,Zhenjiang,Jiangsu 212013);Effects of Different Seeds' Pretreatments on the Growth and Silymarin Content of Silybum marianum Gaertn.[J];Journal of Anhui Agricultural Sciences;2012-15
3 LONG Jie1,2,GAO Hai-yan2,*,CHEN Hang-jun2,*,SONG Li-li2,FANG Xiang-jun2(1.College of Food Science and Technology,Nanjing Agricultural University,Nanjing 210095,China;2.Institute of Food Processing,Zhejiang Academy of Agricultural Science,Hangzhou 310021,China);Research Advance in Storage of Mulberry[J];Storage & Process;2011-03
4 Wang Zhan1,Fu Yujie1,2,Chang Hui1,Zhao Changjun1,Mi Mantian1(1Department of Nutrition and Food Hygiene,Chongqing Key Laboratory of Nutrition and Food Safety,College of Military Preventive Medicine,Third Military Medical University,Chongqing,400038;2School of Pharmacy and Bioengineering,Chongqing University of Technology,Chongqing,400050,China);Inhibitory effect of anthocyanin extract of mulberry fruit on growth of breast cancer MDA-MB-453 cells[J];Journal of Third Military Medical University;2011-10
5 Xie Yan,Chang Wei,Shu Furong,Zhao Changjun,Mi Mantian(Department of Nutrition and Food Hygiene,Chongqing Key Laboratory of Nutrition and Food Safety,College of Military Preventive Medicine,Third Military Medical University,Chongqing,400038,China);Effects of 3,6-dihydroxyflavone on growth and microRNAs expression profile in transplanted tumor of breast cancer MDA-MB-453 cells[J];Journal of Third Military Medical University;2012-02
6 BAO Lijun(College of Agriculture and Life Sciences,Ankang University,Ankang 725000,Shaanxi,China);Ultrasonic Extraction of Proanthocyanidins from Mulberry Fruit[J];Journal of Ankang University;2012-06
7 SU Chao1* XUE Zhong-Min1 JIAO Feng1 CHEN Qi1 SU Wei-Lei2 LIU Hai-Jun1(1Institute of Sericulture and Silk,Northwest A & F University,Yangling Shaanxi 712100,China;2College of Life Sciences,Northwest A & F University,Yangling Shaanxi 712100,China);Breeding of a New Mulberry Variety Hongguo 2 for Fruit Use[J];Science of Sericulture;2012-06
8 KUAI Yuan-Zhang1,2 WU Fu-An1,2*(1Jiangsu University of Science and technology,Zhenjiang Jiangsu 212003,China;2The Sericultural Research Institute,Chinese Academy of Agricultural Sciences,Zhenjiang Jiangsu 212018,China);A Review on Pathogens of Mulberry Fruit Sclerotiniosis and Its Control Technology[J];Science of Sericulture;2012-06
9 Lü Huan1 WEN Yan1 WANG Jun1 XU Xiao-Feng1,2 WU Yu1 JIANG Ming-Zhu1(1School of Biological and Chemical Engineering,Jiangsu University of Science and Technology,Zhenjiang Jiangsu 212018,China;2College of Life Sciences,Nanjing Normal University,Nanjing 210046,China);The Influence of Mulberry Fruit Juice on Proliferation and Apoptosis of Colon Cancer Cell Line SW620[J];Science of Sericulture;2013-02
10 YANG Fang(Department of Agriculture and Life Sciences,Ankang University / Selenium-enriched Food Engineering Laboratory of Shaanxi Province,An'kang 725000,Shaanxi,China);Determination of Selenium in Mulberry from Hanbin District of Ankang[J];Hubei Agricultural Sciences;2012-14
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 Zhao Jin 1 ,Wang Di 2 ,Zhao Hong 2 ,Lin Wei 21.The Fifth Department,Medical College of Northwest Minorities University,Lanzhou,Gansu,730000,P.R.China2.Department of Microbiology and Immunology,Lanzhou Institute of Biological Product,Lanzhou,Gansu,730000,P.R.China;Comparative Study on Apoptosis Indu ction of SMMC7721 and Vero Cells by Clostridium difficile Toxin A[J];Chinese Journal of Cancer;2003-07
2 CHANG Hui1, MI Man-Tian1, GU Yan-Yan1, YUAN Jia-Lin1, LING Wen-Hua2, LIN Hui1 1. Department of Nutrition and Food Hygiene, College of Preventive Medicine, Third Military University, Chongqing Key Laboratory of Nutrition and Food Safety, Chongqing, 400038, P. R. China 2. Department of Nutrition, Public Health School, Sun Yet-sen University, Guangzhou, Guangdong, 510080, P. R. China;Effects of Flavonoids with Different Structures on Proliferation of Leukemia Cell Line HL-60[J];Chinese Journal of Cancer;2007-12
3 WU Yu-Ming SHI Ying XIAO Geng-Sheng XU Yu-Juan ZOU Yu-Xiao YAO Xi-Zhen(The Sericulture and Farm Products Processing Research Institute of Guangdong Academy of Agricultural Sciences,Public Laboratory of Farm Products Processing Technology of Guangdong Province,Guangzhou 510610,China);Studies on Nutrient Constituents in Mulberry Seeds[J];Science of Sericulture;2006-01
4 WANG Zhen-Jiang XIAO Geng-Sheng~* LIAO Sen-Tai LIU Xue-MingTANG Cui-Ming XU Yu-Juan CAO Yang(~(1)The Sericulture & Farm Produce Processing Research Institute of Guangdong Academy of AgriculturalSciences,Guangdong Open Access Laboratory of Agricultural product processing,Guangzhou 510610 China;~(2)Animal Science College,South China Agricultural University,Guangzhou 510642 China);The Study of Antioxidations and Their Correlations With Anthocyanin Contents in Different Varieties of Mulberry[J];Science of Sericulture;2006-03
5 ZHU Xiang-Rui1 FEI Jian-Ming2 YANG Yi-Wen3 YANG Kun1(1College of Animal Sciences,Zhejiang University,Hangzhou 310029,China;2Huzhou Academy of Agricultural Science,Huzhou Zhejiang 313000,China;3Anji Chaolong Sericultural Development Company,Anji Zhejiang 313300,China);Preliminary Investigation on Resveratrol Content in Mulberry Pericarp and Branch[J];Science of Sericulture;2007-01
6 ZHANG Li1 WANG Hua1,2 LIANG Yan-Ying1 TAO Yong-Sheng1(1College of Enology,Northwest A & F University,Yangling Shaanxi 712100,China;2 College of Food Science and Engineering,Northwest A & F University,Yangling Shaanxi 712100,China);Analysis of Volatile Components in Mulberry by Gas Chromatograph-Mass Spectrometer[J];Science of Sericulture;2007-02
7 TIAN Cheng-rui 1,XU Jian-guo 1,2 ,HU Qing-ping 2 (1 Departerment of Food Engineering, Shaanxi Normal University,Xi'an 710062,China;2 College of Life Science, Shanxi Normal University,Linfen Shanxi 041000,China);Conditions for Dynamic Adsorption in the Purification of Mulberry Red Pigment[J];Acta Botanica Boreali-occidentalia Sinica;2005-06
8 TANG Yong1, ZHAO Jing1, XU Jing2, ZHANG Ting1, ZHANG Yan-qi3, MI Man-tian1 (1Department of Nutrition and Food Hygiene, Chongqing Key Laboratory of Nutrition and Food Safty, 3Department of Statistics, College of Preventive Medicine, Chongqing 400038, 2Department of Endocrinology, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China);Evaluation and quantification of total antioxidative capability of fruit by microassay[J];Acta Academiae Medicinae Militaris Tertiae;2008-06
9 CHANG Hui1, MI Man-tian1, GU Yan-yan1, YUAN Jia-lin1, LING Wen-hua2, LIN Hui1 (1Department of Nutrition and Food Hygiene, College of Preventive Medicine, Third Military Medical University, Chongqing 400038; 2Department of Nutrition, Public Health School, Sun Yet-sen University, Guangzhou 510080, China);Effects of anthocyanin-rich extract from black rice on ROS and mitochondrial membrane potential of leukemia HL-60 cells and human lymphocytes[J];Acta Academiae Medicinae Militaris Tertiae;2008-07
10 LIN Hui,MI Man-tian,CHANG Hui(Department of Nutrition and Food Hygiene,College of Military Preventive Medicine,Third Military Medical University,Chongqing 400038,China);3,6-dihydroxyflavone decreases anti-oxidative enzyme activities in human leukemia HL-60 cells via MAPKs signaling pathway[J];Acta Academiae Medicinae Militaris Tertiae;2009-10
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