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Expression of Human Prostate Secretory Protein of 94 Amino Acids Fusion Protein in E.coli and Activity Assay of the Expressed Product

Liu Jianxiang\+*\ Liu Li\ Liu Lizhong *MH Radiobiology,Norman Bethune University of Medical Sciences,Changchun\ 130021 Research Center of Molecular Biology,Airforce Hospital,Beijing 100036  
We had previously cloned the whole length cDNA of human prostate secretory protein of 94 amino acids (PSP94) from normal adult prostatic tissues by RT\|PCR.In this study, we reported expression of human PSP94 fusion protein in E.coli.The chimeric gene coding for 19aa\|PSP94 fusion proteins under control of the P\-L promoter was constructed. The expression was assayed by SDS\|PAGE,which showed that the molecular weight of the 19aa\|PSP94 fusion protein was in accordance with the estimated value of 16.5kD.It was 30% of total bacterial protein and in the form of inclusion body. Through biological activity analysis, 19aa\|PSP94 fusion protein has anti\|prostatic cancer activity.\;
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