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Application of HRP-HBVDNA Probe in Clinic Detection

Lan RongLiang\ Liu Yujing\+*\ Chen Yufang\ Qiao Hong\ Tang Yan (Beijing Biochemical Immune Reagents Center,100012) (*Central Laboratory,People Hospital,Beijing Medical University,100044)  
A simple method for detection of HBV DNA in sera is described in this thesis.A direct enzyme labeled HRP\|HBV DNA probe was constructed according to Renz et al.After hybridization of the probe with target DNA in sera immobilized on nitrocellulose membrane, the result could be observed by the method of chemiluminescence auto\|photographic detection.\;The sensitivity is as low as 0.1pg of target DNA,comparable with that of isotope probe,HBV DNA was detected in 63 sera of HBsAg,HBeAg and Anti\|HBc ELISA positive and 24 of HBsAg,Anti\|HBc positive,HBeAg negative with HBV DNA positive rates of 100% (63) and 58% (14),respectively;50 sera of HBsAg ELISA negative and ALT normal with HBV DNA all negative by the probe.\;The result shows that this method is of great value to popularize.\;
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