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《Progress In Biotechnology》 2004-01
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Expression of Truncated Region of S Genome Segment of Hantaan Virus in E.coli and Preliminary Use of This Recombinant Protein

WANG Bin\ ZHAO Bai\|hui\ QIAN Dong\|meng\ YAN Zhi\|yong (Key laboratory of medicine and bio technology Qingdao Medical College\ Qingdao\ 266021, China)  
Objective\ Cloning and expression of Hantaan virus S gene truncated region(SA) and using it in rapid diagnosis of HFRS.Methods Obtained SA by RT PCR from the HFRS patient peripheral blood and cloned into GST fusion protein expression vector PGEX4T 2 by PCR and restriction digest methods,the recombinant protein was examined by immunoblot with polycloned antibodies.Results The molecular weights of SA was 49000 kDa and mainly expressed as inclusion body in Escherichia coli .Western blot analyses demonstrated that this recombinant protein presented the specific antigenicity\ Conclusion The prokaryotic expression of portions of the NPs of this specific Hantaan virus could be used to generate readily an efficiency in diagnostic assays for HFRS.;
【Fund】: 山东省重点医药科技攻关计划资助项目 (CX0 2 10 4)
【CateGory Index】: R346
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