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《Progress In Biotechnology》 2004-01
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Preparation of Affinity Chromatographic Adsorbents for Pectinases Purification

JIA Yue\+2\ BAI Zhi\|hui\+1\ GONG Ai jun\+2\ ZHANG Hong xun\+1 (1 Research center for Eco\|Environmental Sciences, CAS, Beijing\ 100085,China) (2 Beijing University of Sciences and Technology, Beijing\ 100083,China)  
Suger beet pulp (SBP)powder, pectin and sodium alginate (SA) were cross\|linked with epichlorohydrin respectively in an alkaline condition.Such preparations were used as the affinity chromatographic adsorbents for the separation and purification of pectinase from Penicillium oxalicum and Aspergillus niger. The results showed that the affinity chromatographic adsorbent prepared by SBP powder with less than 60 sieve mesh has better character than others for pectinase purification. SBP adsorbent was stable, while SA adsorbent resulted in unstable flow of mobile phase because its larger dilatability.;
【Fund】: 国家“8 63”计划资助项目 ( 2 0 0 1AA2 460 14 );; 科学院知识创新工程项目 (KZCX1 SW 19)
【CateGory Index】: TQ424
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【References】
Chinese Journal Full-text Database 2 Hits
1 DAI Ru-wen,HE Fu-yuan,LUO Jie-ying,PI Feng-juan,FU Jin,WU De-zhi(TCM University of Hunan,Changsha,Hunan 410208,China);Study on affinity of non-component carrier with counter-zuojin components[J];Journal of Traditional Chinese Medicine University of Hunan;2008-05
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【Citations】
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1 WU Huamei; Qing Huai; Xiang Ming; Li Zhuyan (Instituteof Biology, Guizhou Academy of Sciences, Guiyang 550009) Zhou Jian (Department of Horticulture,Guizhou Agricultural College, Guiyang 550025);THE PURIFICATION AND CHARACTERIZATION OF PECTINASES FROM CLADOSPORIUM HERBARUM AND ITS MUTANS[J];GUIZHOU SCIENCE;1997-03
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1 AO Guang-hui~1,LIU Ya-qiu~2,LI Bo-jun~3(1.Department of Chemistry and Life Science,Neijiang Teachers College,Neijing,Sichuan 641112,China;2. Dazu Middle School,Dazu,Chongqing 402300,China;3.Yaan Middle School,Yaan,Sichuan 625000,China);The Molecular Markers Linked to Plant Genetic Diversity and Population Ecology[J];Journal of Neijiang Teachers College;2005-04
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1 Li Zuming1,2,3 Zhang Hongxun2 Bai Zhihui2 Li Hongyu3(1College of Arts and Sciences of Beijing Union University,Beijing 100191;2 Research Center for Eco-Environmental Sciences,Chinese Academy of Sciences,Beijing 100085;3Teacher's College of Beijing Union University,Beijing 100011);Advance in Microbial Pectinases Research[J];Biotechnology Bulletin;2010-03
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