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《Progress In Biotechnology》 2004-01
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Preparation of Affinity Chromatographic Adsorbents for Pectinases Purification

JIA Yue\+2\ BAI Zhi\|hui\+1\ GONG Ai jun\+2\ ZHANG Hong xun\+1 (1 Research center for Eco\|Environmental Sciences, CAS, Beijing\ 100085,China) (2 Beijing University of Sciences and Technology, Beijing\ 100083,China)  
Suger beet pulp (SBP)powder, pectin and sodium alginate (SA) were cross\|linked with epichlorohydrin respectively in an alkaline condition.Such preparations were used as the affinity chromatographic adsorbents for the separation and purification of pectinase from Penicillium oxalicum and Aspergillus niger. The results showed that the affinity chromatographic adsorbent prepared by SBP powder with less than 60 sieve mesh has better character than others for pectinase purification. SBP adsorbent was stable, while SA adsorbent resulted in unstable flow of mobile phase because its larger dilatability.;
【Fund】: 国家“8 63”计划资助项目 ( 2 0 0 1AA2 460 14 );; 科学院知识创新工程项目 (KZCX1 SW 19)
【CateGory Index】: TQ424
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2 PENG Xiawei 1, 2**, XIE Xiangming 1, BAI Zhihui 2 & ZHANG Hongxun 2, 3**( 1College of Biological Science and Technology, Beijing Forestry University, Beijing 100083, China)( 2Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China)( 3Graduate University of the Chinese Academy of Sciences, Beijing 100049, China);Purification and Induced Resistance of Pectinases from Penicillium oxalicum BZH-2002[J];Chinese Journal of Applied & Environmental Biology;2006-06
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1 WU Huamei; Qing Huai; Xiang Ming; Li Zhuyan (Instituteof Biology, Guizhou Academy of Sciences, Guiyang 550009) Zhou Jian (Department of Horticulture,Guizhou Agricultural College, Guiyang 550025);THE PURIFICATION AND CHARACTERIZATION OF PECTINASES FROM CLADOSPORIUM HERBARUM AND ITS MUTANS[J];GUIZHOU SCIENCE;1997-03
2 GU Hong-yan, QI Hong-yan, ZHANG Hong-xun (Research Center for Eco-envionmental Sciences, Chinese Academy of Sciences, Beijing 100085, China);Fermentation Conditions of Pectinase by Mucor mucedo[J];The Chinese Journal of Process Engineering;2002-03
3 Gang Jie Liu Xiaolan(Qiqihar University Industry Institute)Li Caiqin   (Qiqihar City Beidacang Brewery)     Peng Kai T  (Qiqihar Universty Industry Institute);Determination of Pectinase Activity by Spectrophotometry[J];JOURNAL OF QIQIHAR UNIVERSITY(NATURAL SCIENCE EDITION);1998-01
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【Secondary References】
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1 Li Zuming1,2,3 Zhang Hongxun2 Bai Zhihui2 Li Hongyu3(1College of Arts and Sciences of Beijing Union University,Beijing 100191;2 Research Center for Eco-Environmental Sciences,Chinese Academy of Sciences,Beijing 100085;3Teacher's College of Beijing Union University,Beijing 100011);Advance in Microbial Pectinases Research[J];Biotechnology Bulletin;2010-03
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