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《China Biotechnology》 2006-03
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Advances in Study on Lipase Bio-imprinting

DONG Bin1 SONG Xi-jin1 WANG Jie2 (1 Department of Chemical & Biochemical Engineering, Zhejiang University Hangzhou 310027, China) (2 Department of Chemistry, Zhejiang University Hangzhou 310027, China)  
Lipase is a kind of widely used hydrolase. Bio-imprinting is a new technology , it induces the lipase to exhibit exciting catalytic ability in nonaqueous solvents, such as higher activity and stability, and enhances lipase’s industrial use as bio-catalysts. The characteristic of the lipase’s structure, the process and the mechanism of the bio-imprinting were introduced. Influencing factors such as the selective principle of different kinds of bio-imprinting templates and the function of lyoprotectant through the process of the bio-imprinting were discussed. Meanwhile, the condition of the bio-imprinting, the reaction catalyzed by bio-imprinted lipase and the results were concluded. Finally, the problem and the prospect of the bio-imprinting technology were mentioned.
【Fund】: 国家自然科学基金资助项目(20276067)
【CateGory Index】: Q55-33
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