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《China Biotechnology》 2009-01
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Prokaryotic Expression and Acetylation Assays of Histone Acetyltransferase PCAF

LI Wei-zhong ZHANG Dan-gui ZENG Jun WANG Ge-fei CHEN Xiao-xuanCHEN You-ying LI Kang-sheng(Department of Microbiology and Immunology,Shantou University Medical College,Shantou 515031,China)  
P300/CBP-associated factor(PCAF),an important member of histone acetyltransferase family(HATs) within eukaryotic cells,is capable of inducing the acetylation of histone,promoting the transcription of specific genes and involving in many biological effects.In the present study,full-length cDNA of PCAF was inserted into plasmid pGEX-5x-1,then the soluble protein GST-PCAF was expressed in E.coli BL21(DE3) after the optimization of inducing conditions.The recombinant protein was further purified with affinity chromatography and tested the activity by in vitro acetylation assays.High efficient PCAF protein produced by this method could serve for the study on the role of PCAF in gene regulation and the interaction between PCAF and other proteins.
【Fund】: 国家自然科学基金(30571674);; 广东省自然科学基金(05008347)资助项目
【CateGory Index】: Q786
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