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《China Biotechnology》 2009-01
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Methods for Regeneration of Five Kinds of SPR Sensor Chips and Their Applications

OU Hui-chao1,2 JIANG Hao1 ZHOU Hong-min1 LI Lei-ke1 BAI Lin-jing1SONG Cun-xian2 LUO Zhao-feng1(1 Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences,University of Science & Technology of China,Hefei 230027,China)(2 The Tianjin Key Laboratory of Biomaterial Research,Institute of Biomedical Engineering,Peking Union Medical College &Chinese Academy of Medical Sciences,Tianjin 300192,China)  
The biosensor based on the principle of surface plasmon resonance(SPR),is a surface sensitive optical device for monitoring biomolecular interactions at the sensor surface in real time without any labeling.It is being used in a wide variety of areas such as proteomics,drug discovery,clinical diagnosis,food analysis,environmental monitoring and so on.The sensor chi Pis the core of the Biacore instrument.Since sensor chips can now only be purchased from Biacore AB(Uppsala,Sweden),which makes them expensive,many instruments are either used at a low rate or sleeping.Simple and cost-effective methods for the regeneration of five kinds of sensor chips for the Biacore instrument,which include the J1,C1,CM5,SA and NTA sensor chip,were described.And several applications of these regenerated sensor chips were listed.Through the improvement and optimization of these methods in the last four years,the regenerated sensor chi Pcan achieve the same quality as the Biacore sensor chip.The wide use of these regeneration methods will be highly beneficial to the application and development of SPR technology in a wider variety of areas.
【Fund】: 国家“863”计划(2006AA06Z406);; 国家自然科学基金(30727001)资助项目
【CateGory Index】: Q819
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