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《China Biotechnology》 2009-01
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A Novel Method to Detect a New Biopolymer Material—Poly-γ-Glutamic Acid

ZHANG Chao CHE Cheng-chuan QU Xiao-meng YANG Ge(College of Life Science Qufu Normal University,Qufu 273165,China)  
A turbid solution was formed through the reaction between γ-PGA and cetylpyridinium chloride(CPC) and the turibidity was measured by using ultraviolet-visible spectroscopy at 680nm.The linearity between the concentration of γ-PGA and its absorbance,the stability,repeatability and recovery of the method were studied.Through the reaction of γ-PGA with CPC,a homogeneous turbid solution was formed.The turbid solution was stable in 3h under proper pH value and ion strength,the absorbance of the solution at 680nm had a good linear relationshi Pwith the concentration of γ-PGA in the range 12.5~50μg/ml(R2=0.9939).The recovery was within the range of 86%~99.75%(n=5).The relative standard division of the method for determining γ-PGA at the concentrations of 5,10 and 40μg/ml were 0.14%,0.23%和0.025% repeatability.The turbidmetric method has advantages of convenience,simplicity and good repeatability and can be used to control the quality of γ-PGA and its products.
【Fund】: 中国科学院微生物研究所微生物前期开发国家重点实验室开放课题资助项目(SKLMR-080606)
【CateGory Index】: Q51-3
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